Adhesión a dentina parte I: características del sustrato y sistemas adhesivos / Dentin bonding part I: substrate characteristics and bonding systems

La Odontología Restauradora contemporánea no puede concebirse sin la adhesión de los materiales restauradores a las estructuras dentarias. En mu-chos procedimientos restauradores, no sólo el esmal-te sino también la dentina se encuentra involucrada, por lo tanto, la adhesión a esta última juega un rol cla-ve en el éxito clínico. No obstante, todavía supone un gran desafío lograr una adhesión estable y predeci-ble en el tiempo. El propósito del presente artículo es analizar las características principales de la dentina como sustrato adhesivo y describir los sistemas ad-hesivos actuales y sus mecanismos de acción (AU)

Contemporary Restorative Dentistry cannot be conceived without the adhesion of restorative materials to dental structures. In many restorative procedures not only the enamel, but also the dentin is involved, therefore dentin bonding plays a key role in their success. However, it is still a great challenge to achieve stable and predictable dentin bonding over time. The aim of this article is to analyze the main characteristics of dentin as an adhesive substrate and to describe current adhesive systems and their bonding mechanisms (AU)

Candida albicans biofilms and MMA surface treatment influence the adhesion of soft denture liners to PMMA resin

The effect of Candida albicans biofilms and methyl methacrylate (MMA) pretreatment on the bond strength between soft denture liners and polymethyl methacrylate (PMMA) resin was analyzed. Specimens were prepared and randomly divided with respect to PMMA pretreatment, soft liner type (silicone-based or PMMA-based), and presence or absence of a C. albicans biofilm. Samples were composed of a soft denture liner bonded between two PMMA bars. Specimens (n = 10) were incubated to produce a C. albicans biofilm or stored in sterile PBS for 12 days. The tensile bond strength test was performed and failure type was determined using a stereomicroscope. Surface roughness (SR) and scanning electron microscopy (SEM) analysis were performed on denture liners (n = 8). Highest bond strength was observed in samples containing a silicone-based soft liner and stored in PBS, regardless of pretreatment (p < 0.01). Silicone-based specimens mostly underwent adhesive failures, while samples containing PMMA-based liners predominantly underwent cohesive failures. The silicone-based specimens SR decreased after 12 days of biofilm accumulation or PBS storage, while the SR of PMMA-based soft liners increased (p < 0.01). The PMMA-based soft liners surfaces presented sharp valleys and depressions, while silicone-based specimens surfaces exhibited more gentle features. In vitro exposure to C. albicans biofilms reduced the adhesion of denture liners to PMMA resin, and MMA pretreatment is recommended during relining procedures.

Effect of incorporation of 2-tert-butylaminoethyl methacrylate on flexural strength of a denture base acrylic resin

Polymethyl methacrylate (PMMA) resins have commonly been used as a denture base material. However, denture bases may act as a reservoir for microorganisms and contribute to oral diseases in denture wearers. It is hypothesized that the 2-tert-butylaminoethyl methacrylate (TBAEMA) incorporated to acrylic resins should have antimicrobial activity related to the presence of amino groups on acrylic resin surface. OBJECTIVES: The objectives of this study were to evaluate the presence of amino groups on acrylic resin surface and the influence on flexural strength after incorporation of TBAEMA. MATERIAL AND METHODS: Six groups were divided according to the concentration of TBAEMA incorporated to acrylic resin (Lucitone 550): 0, 0.5, 1.0, 1.5, 1.75 and 2 percent. Specimens surface were evaluated by Electron Spectroscopy for Chemical Analysis (ESCA) to detect the presence of amino groups, represented by nitrogen ratios. Flexural strength of the specimens was tested and results were analyzed by ANOVA and Tukey's test (α=0.05). RESULTS: Different nitrogen ratios were observed on specimen surfaces: 0, 0.13, 0.74, 0.66, 0.92 and 0.33 percent for groups 0, 0.5, 1.0, 1.5, 1.75, and 2 percent, respectively. Significant differences were found for flexural strength (p<0.001). The mean flexural strength values were 98.3±3.9, 93.3±3.2, 83.9±2.1, 82.8±5.2, 71.2±5.1 and 17.3±3.2 MPa for groups 0, 0.5, 1.0, 1.5, 1.75, and 2 percent, respectively. CONCLUSION: Within the limitations of this study, the incorporation of TBAEMA results in the presence of the potentially antimicrobial amino groups on specimen surfaces, but affect the flexural strength, depending on the concentration of TBAEMA.

Glutaraldehyde release from heat-polymerized acrylic resins after disinfection and chemical and mechanical polishing

This study evaluated the release of glutaraldehyde from heat-polymerized acrylic resins subjected to disinfection followed by chemical and mechanical polishing. Ninety disc-shaped specimens (15 x 4 mm), 30 per resin (Lucitone 550, QC-20 and Classico), were made and assigned to 2 groups according to the type of polishing. One side of each specimen was not polished and the other was either mechanically (n=45) or chemically (n=45) polished, and immersed in water at 50°C for 1 h to allow the release of intrinsic substances and then kept in distilled water for 7 days. The specimens were disinfected by immersion in 2% glutaraldehyde for 10 min. After this period, 3 specimens from each group were immersed in water for 15, 30, 60, 120 and 240 min. For the 15-, 30-, 60-min immersions, 4 water exchanges were done at the end of period. High performance liquid chromatography (HPLC) was used to detect and quantify the glutaraldehyde released after each period. Data were analyzed statistically by two-way ANOVA and multiple comparisons were done by Tukey’s and Scheffé’s tests (α=0.05). No glutaraldehyde release was observed from the specimens with chemical polishing at any of the immersion periods, while the mechanically polished specimens released glutaraldehyde. In the groups with water exchanges, Lucitone released more disinfectant in the 15-min period (0.040 μg/mL), Classico in the 30-min (0.021 μg/mL) and 60-min (0.018 μg/mL) periods, and QC-20 the same amount (-1.760 μg/mL) in all periods. In the groups without water exchanges, Lucitone released the highest amount of disinfectant (-1.370 μg/mL), differing significantly from QC-20 (0022 g/mL) and Classico (0019 g/mL), which were similar. The findings of this showed that chemically polished specimens from the 3 resin brands did not release glutaraldehyde after different periods of immersion, while glutaraldehyde release was observed from the mechanically polished specimens, especially from those made of Lucitone resin.

Este estudo determinou a liberação de glutaraldeído de resinas acrílicas termopolimerizáveis submetidas a polimento químico e mecânico e desinfetadas. Noventa corpos-de-prova circulares (15 x 4 mm), 30 de cada tipo de resina (Lucitone, QC-20 e Clássico), foram confeccionados e divididos em 2 grupos referentes ao tipo de polimento. Um dos lados de cada corpo-de-prova não foi polido e o outro foi polido mecanicamente (n=45) ou quimicamente (n=45), e imersos em água aquecida a 50°C por 1 h para liberação de substâncias intrínsecas e mantidos em água destilada por 7 dias. A seguir era realizada a desinfecção por imersão em solução de glutaraldeído a 2% por 10 min. Decorrido este período, três corpos-de-prova de cada grupo eram imersos em água por 15, 30, 60, 120 e 240 min. Nos períodos de 15, 30 e 60 min foram realizadas até 4 trocas de água após cada período. As amostras eram analisadas por meio de cromatografia líquida de alta eficiência (HPLC) após cada período. Os dados foram analisados estatisticamente pela Análise de Variância e testes complementares de Tukey e Scheffé (α=0,05). Os corpos-de-prova com polimento químico, de todas as marcas comerciais de resina, não liberaram glutaraldeído em qualquer um dos períodos de imersão em água, enquanto os com polimento mecânico liberaram. Nos grupos com trocas de água, a resina Lucitone liberou maior quantidade de desinfetante nas trocas de 15 min (0,040 μg/mL), a resina Clássico nas de 30 (0,021 μg/mL) e 60 min (0,018 μg/mL) e a QC-20 liberou a mesma quantidade (-1,760 μg/mL), em todos os períodos de imersão em água. Nos grupos sem trocas de água, a resina Lucitone liberou maior quantidade de desinfetante (-1,370 μg/mL), sendo diferente estatisticamente das resinas QC-20 (0,022 μg/mL) e Clássico (0,019 μg/mL), que são similares. Pelos resultados conclui-se que corpos-de-prova polidos quimicamente, das três marcas comerciais de resina, não liberaram glutaraldeído após os diferentes períodos de imersão. Contudo, nos corpos-de-prova polidos mecanicamente houve liberação do desinfetante, com Lucitone liberando maior quantidade em relação às demais resinas estudadas.

Influence of the processing technique on the flexural fatigue strength of denture base resins: An in vitro investigation.

Background : Though acrylic resins possess many desirable properties, denture fracture due to flexural fatigue or impact failure is a common problem. One major factor influencing the flexural fatigue strength of denture base resins is the processing technique used. Aim: To measure the flexural fatigue strength of denture base resins polymerized using short and long curing cycles using water bath, pressure cooker, and microwave polymerization techniques. Materials and Methods: Flexural fatigue strength of 60 samples (n=10) were measured using a cyclic 3-point loading method on a dynamic universal testing machine. Data were analyzed using a Student 't' test. Results : Comparative evaluation using Student's 't' test of mean flexural fatigue strength of samples processed by water bath processing (660.6) and the microwave technique (893.6) showed statistically significant (P <0.01) result with microwave processing being higher. Comparison of water bath (660.6) and pressure cooker (740.6) processing and microwave (893.6) and pressure cooker (740.6) processing using Student's 't' test was not statistically significant (P >0.05). In the intra-group analysis, it was found that there was statistically significant difference in samples processed using the short and long curing cycle, the latter being better in all groups, P-values being <0.05, <0.001, and <0.001 for water bath, microwave, and pressure cooker polymerization techniques, respectively. Conclusion : The polymerization procedure plays an important role in influencing the flexural fatigue strength of denture base resins, and the microwave long curing processing technique produced denture bases with highest flexural fatigue strength.

Infrared spectroscopy: a tool for determination of the degree of conversion in dental composites

Infrared spectroscopy is one of the most widely used techniques for measurement of conversion degree in dental composites. However, to obtain good quality spectra and quantitative analysis from spectral data, appropriate expertise and knowledge of the technique are mandatory. This paper presents important details to use infrared spectroscopy for determination of the conversion degree.